Venture - Undergraduate Research Database
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Genetic characterization and analysis of novel CRISPR-generated animal models of cancer

The Sempere lab has generated novel mouse and rat cancer models using easi-CRISPR tools. We need a talented student to characterize in detail the generated genetic alterations and to determine whether engineered regulatory elements operate as intended.

This project requires detailed genetic characterization of the generated genetic alterations to confirm that the designed genetic regulatory elements were appropriately targeted in the genome. Different molecular genetic tools will be used to characterize the location and structure of introduced regulatory elements in relation to endogenous gene locus or region as well as to determine the sequence of individual transgenic elements and flanking genomic sequence. We have 8 different new genetic engineered animal strains (two rat and six mouse models) that will need to be studied and characterized in a similar manner.

The student will need to learn and perform in routine basis the following tasks to achieve the goals of this project:

1) Handling tubes and liquids with manual, automated and multi-channel pipettes.

2) Standard, high-fidelity, long-range, and single-allele PCR.

3) DNA electrophoresis, gel imaging and documentation.

4) Molecular cloning, bacterial transformation and culture, colony identification.

5) Sanger and deep sequencing, sequencing data analysis.

6) Electronic of DNA sequencing data and animal record keeping.

The PI or a senior lab member will show student how to perform these tasks and provide guidance during performance, analysis and interpretation of results. The student will also have the opportunity to participate in the design of new screening strategies and generation of new animal models.


Students who are interested in genetics, cancer biology, molecular biology, cellular biology, and/or biochemistry are encouraged to apply.

Student should have good organizational skills, and a basic understanding of Mendelian genetics and molecular genetic tools.

GPA of 3.5 or higher and at least 8-10hrs/week time commitment in the lab are required for consideration.

To apply send a cover letter with your scientific interests, career goals and why you want to work with us, and an updated CV to

Project Coordinator

lorenzo sempere ( Z41765104 )
Posted: 18-10-12
no compensation
Start Date: 18-10-12
End Date: 18-10-28

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