Venture - Undergraduate Research Database
  • This entry is EXPIRED

Genetic characterization and analysis of novel CRISPR-generated animal models of cancer

The Sempere lab has generated novel mouse and rat cancer models using easi-CRISPR tools. We need a talented student to characterize in detail the generated genetic alterations and to determine whether engineered regulatory elements operate as intended.

This project requires detailed genetic characterization of the generated genetic alterations to confirm that the designed genetic regulatory elements were appropriately targeted in the genome. Different molecular genetic tools will be used to characterize the location and structure of introduced regulatory elements in relation to endogenous gene locus or region as well as to determine the sequence of individual transgenic elements and flanking genomic sequence. We have 8 different new genetic engineered animal strains (two rat and six mouse models) that will need to be studied and characterized in a similar manner.

The student will need to learn and perform in routine basis the following tasks to achieve the goals of this project:

1) Handling tubes and liquids with manual, automated and multi-channel pipettes.

2) Standard, high-fidelity, long-range, and single-allele PCR.

3) DNA electrophoresis, gel imaging and documentation.

4) Molecular cloning, bacterial transformation and culture, colony identification.

5) Sanger and deep sequencing, sequencing data analysis.

6) Electronic of DNA sequencing data and animal record keeping.

The PI or a senior lab member will show student how to perform these tasks and provide guidance during performance, analysis and interpretation of results. The student will also have the opportunity to participate in the design of new screening strategies and generation of new animal models.

Qualifications

Students who are interested in genetics, cancer biology, molecular biology, cellular biology, and/or biochemistry are encouraged to apply.

Student should have good organizational skills, and a basic understanding of Mendelian genetics and molecular genetic tools.

GPA of 3.5 or higher and at least 8-10hrs/week time commitment in the lab are required for consideration.

To apply send a cover letter with your scientific interests, career goals and why you want to work with us, and an updated CV to semperel@msu.edu.

Project Coordinator

lorenzo sempere ( Z41765104 )
5173553982
Posted: 18-10-12
Category:
no compensation
Start Date: 18-10-12
End Date: 18-10-28

Students: Find a Project
  • By Unit

    Explore projects sorted by college or unit.

  • By Category

    Explore projects sorted by broad categories.

  • By Keyword

    Not sure of where to start or what your research interests might be?  The keywords section can assist you in your search.  Type a general word that describes your research area of interest.

  • Paid Opportunities

    Search for paid opportunities

  • Creditable Positions

    Search for opportunities for which credit is available.

Research Mentors: Post a Project
  • Create a new project

    To post a project, you will need the following information:

    Project title
    Keywords for searching
    Student qualifications/requirements
    Brief project overview
    In-depth project details

  • Refine or manage your posted projects

    Click here to access your project management dashboard. The dashboard allows project coordinators to post, edit, and update projects.

Newest Research Opportunities

Conservation Criminology

Participate in applied conservation social science research integrating conservation biology, criminology and risk and decision science
Posted: 18-11-09
Category:
no compensation
Start Date: 18-11-09
End Date: 19-04-30

Organic Energy Storage Lab Summer Intern 2019

The OESLaboratory at the MSU Bioeconomy Institute in Holland, MI has openings for summer 2019 interns. The research covers energy storage, alternative energy, organic synthesis, analytical chemistry, electrochemistry and device design.
Posted: 18-11-07
Category:
Compensation:
Start Date: 18-11-07
End Date: 19-02-15

Coding of Spanish speech files

This project examines how the pronunciation of [s] by Spanish speaking parents influences the rate of acquisition of plural morphology (also realized as [s])
Posted: 18-09-11
Category:
Compensation:
Start Date: 18-09-11
End Date: 18-12-16

Questions? Contact the Undergraduate Research Office at 517-355-7635 or
© 2009 Michigan State University Board of Trustees 312 Administration Building East Lansing, MI 48824 517-353-5380
login
Designed and developed by Redhead Design Studio
Michigan State University